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Composition of physiological solutions used
| NaCl | KCl | CaCl2 | MgCl2 | Hepes | Glucose | Sodium gluconate | Calcium gluconate | Potassium gluconate | Magnesium gluconate | NMDG-Cl | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Iso Hanks | 140 | 2.5 | 1.2 | 0.5 | 10 | 5 | — | — | — | — | — |
| Cl− free | — | — | — | — | 10 | 5 | 140 | 10 | 2.5 | 0.5 | — |
| K+ free | 140 | — | 1.2 | 0.5 | 10 | 5 | — | — | — | — | — |
| Na+ free | — | 2.5 | 1.2 | 0.5 | 10 | 5 | — | — | — | — | 140 |
All quantities are given in millimolar. Solutions were fixed to pH 7.4 with Tris, and osmolarity of 300 mosmol kg−1 using d-mannitol
