TABLE 3.
Recombinant formation in crosses between Hfr GM2807 (dam-16::Kan) and pMQ430/GM7323 (ΔruvABC::Cam Strr)a
| % Arabinose | Recombination frequency |
|---|---|
| None | 5 (Cams UVr) |
| 0.2 | 1,250 (Camr UVs) |
| 0.02 | 4 (Cams UVr) |
| 0.002 | 2 (Cams UVr) |
Bacteria were mated for 60 min and then plated for Kanr Strr recombinants on plates with various concentrations of arabinose. The numbers of recombinants in 50 μl of mating mixture are shown. The Cams UVr recombinants arise from the transfer of the wild-type ruvA, -B, and -C genes from the donor.