Figure 2. Increasing calcium influx by changing pulse duration from 10 to 40 ms did not prevent rapid cessation of exocytosis during a train in Rab3AQ81L-expressing cells.

A, representative capacitance response (C_m) recorded in a bovine adrenal chromaffin cell infected with control adenovirus (GFP–IRES–β-galactosidase), during stimulation with a train of 40 ms depolarizations (20 pulses, −90 mV to +20 mV, 200 ms interval between pulses). B, representative C_m recorded in a cell infected with Rab3AQ81L adenovirus (Rab3AQ81L–IRES–GFP), during stimulation with a train of 40 ms depolarizations. Insets, first and last inward currents (I_Na,Ca). C, average C_m response (ΔC_m) for each pulse of the train in control and Rab3AQ81L-expressing cells. D, average calcium influx for each pulse of the train. n= 10 and 9 cells for control and Rab3AQ81L, respectively. [Ca²⁺]_o= 5 mmol l⁻¹. Error bars are ±s.e.m.