Figure 8. Expression of Rab3AQ81L inhibits poststimulus exocytosis, but endocytosis is similar to that of untreated cells.

A, representative capacitance response (C_m) to a single 320 ms depolarization (−90 mV to +20 mV), recorded in a control cell infected with GFP–IRES–β-galactosidase adenovirus. In this response, the amount of poststimulus exocytosis (bracket) is greater than the C_m jump (capacitance increase immediately after gap in trace). B, representative C_m response evoked by a 320 ms depolarization in a cell infected with Rab3AQ81L–IRES–GFP adenovirus. C, C_m response evoked by a 160 ms depolarization in an untreated cell, chosen to match the amplitude of the response shown in B. The red traces represent non-linear least squares fit of the capacitance decay using a mono-exponential function (see Methods).