Figure 1. Western blot showing the expression of three human Na⁺,K⁺-ATPase α subunit isoforms in transduced mpkCCD_cl4 cells.

Cell lysates from mpkCCD_cl4 wild-type cells (lanes 2, 4 and 6) and mpkCCD_cl4 cell lines expressing hα1_myc (lane 1), hα2 (lane 3), and hα3 subunit (lane 5) were probed with anti-myc (lane 1 and 2), anti-α2 (lane 3 and 4) or anti-α3 (lane 5 and 6) antibody. Numbers on the left are kDa. The α subunits appear as ∼110 kDa bands. The band at ∼58 kDa in lanes 5 and 6 is unspecific.