Table 4. Effect of MβCD on several parameters.
| Control | n1 | MβCD | n2 | Decrease | |
|---|---|---|---|---|---|
| Surface (μm2) | 10773 ± 510 | 21 | 10106 ± 533 | 36 | Not significant |
| Capacitance (pF) | 278 ± 14 | 52 | 209 ± 12 | 57 | 25% |
| IT (nA) | −549 ± 63 | 38 | −615 ± 56 | 40 | Not significant |
| IL (nA) | −2.58 ± 0.18 | 38 | 0.85 ± 0.09 | 40 | 67% |
| Gmax (nS) | 58.8 ± 4.5 | 32 | 34.5 ± 2.9 | 20 | 41% |
| Gmax (pS pF−1) | 204 ± 13 | 32 | 169 ± 17 | 20 | 17% |
| Qmax (fC) | 1.30 ± 0.13 | 14 | 0.95 ± 0.11 | 17 | 27% |
| Qmax (fC pF−1) | 5.58 ± 0.42 | 14 | 4.74 ± 0.49 | 17 | 15% |
| Fmax | 4.7 ± 0.3 | 10 | 2.8 ± 0.5 | 9 | 40% |
All the parameters were calculated as described in Methods. IT is the amplitude of T-type Ca2+ current at −20 mV, IL is the peak of the IL–V curve, Gmax is the maximum conductance for L-type Ca2+ current, Qmax is the maximum amount of evoked charge movement and Fmax is the maximum variation of fluorescence in response to a depolarization. Averages of Gmax in pS pF−1 and Qmax in fC pF−1 have been calculated by dividing the value of Gmax or Qmax of each studied cell by it's own capacitance. The percentage reduction is indicated when the values obtained for control and MβCD-treated cells were significantly different (P < 0.05, Mann–Whitney test).