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Cells were seeded and cultured for 48 h in isotonic (0.3osmol (kg H2O)−1) medium before being transferred to a hypertonic (0.5, 0.6 or 0.7osmol (kg H2O)−1) medium. Osmolality was adjusted with sucrose. Culture was then continued for the indicated times and cell growth estimated by measurement of the amount of cell protein. Mean values (±s.d.) from three measurements are given. ○, 0.3; □, 0.5; •, 0.6; ▪, 0.7osmol (kg H2O)−1.
