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Cells were incubated for 16 h in medium of 0.4osmol (kg H2O)−1 and then transferred to medium of 0.3osmol (kg H2O)−1 for the indicated time. The cellular content of ninhydrin-specific solutes was then measured (□). In replicate incubations, radioactively labelled betaine (▪) and myo-inositol (•) were used to monitor their cellular concentrations. Mean values (±s.d.) from three measurements are given.
