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Cells were incubated for 16 h in medium of 0.4osmol (kg H2O)−1 and then transferred to medium of 0.3osmol (kg H2O)−1 for the indicated time, as in Figs 5 and 6. Control cells (C, open bar) were incubated in isotonic (0.3osmol (kg H2O)−1) medium. The cellular content of HSP70 was then monitored by Western blotting (upper panel) and is expressed in the histogram relative to the actin content of the sample (lower panel).
