Figure 6. Effects of 1μm rotenone on resting membrane potential and [Ca²⁺]_i in a type I cell.

A, perforated patch recording of membrane potential in a type I cell. The asterisk denotes a switch from voltage recording (current clamp) mode to voltage clamping the membrane potential to −70 mV. Application of rotenone causes an abrupt membrane depolarization. B, simultaneous recording of [Ca²⁺]_i (using indo-1) from the same cell; note the rapid fall in [Ca²⁺]_i upon on voltage clamping the cell to −70 mV.