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. 2003 Aug;185(16):4908–4919. doi: 10.1128/JB.185.16.4908-4919.2003

FIG. 5.

FIG. 5.

CpxR affects icmR mRNA level. RNA was purified from stationary-phase cultures of the L. pneumophila wild-type strain (JR32), the cpxR insertion mutant (OG2002), and the cpxR insertion mutant containing the complementing plasmid pOG-R-cpx3 (OG2002/cpxR). The upper part of the figure shows the icmR mRNA levels that were analyzed by Northern hybridization, using the icmR gene as a probe. The arrow indicates the icmR mRNA signal (about 620 bp); the size maker is indicated to the right. The lower part of the figure shows equal loadings of samples (10 μg) demonstrated by ethidium bromide staining of rrnA (23S and 16S) (indicated by arrows) in the agarose gel prior to transfer.