TABLE 1.
Plasmids used in this study
| Plasmid | Feature(s) | Reference or Source |
|---|---|---|
| pBR322 | rep (pMB1) rop Apr Tcr | 3 |
| pET-15b | oriR (ColE1) Apr, pT7, N-terminal His6 tag | Novagen |
| pGS-lac-02 | pAB-1 with a promoterless lacZ gene | 16 |
| pGS-reg-RP1 | pOG-R125 containing a mutation in the promoter of icmR | This study |
| pGS-reg-RP2 | pOG-R125 containing a mutation in the promoter of icmR | This study |
| pLAW344 | sacB MCSaoriT (RK2) CmroriR (ColE1) Apr | 47 |
| pMMB207 | RSF1010 derivative, IncQ CmroriT MCS | 29 |
| pOG-BCC2 | E. coli cpxR in pOG-BRR3 | This study |
| pOG-BRM1 | pOG-R125 containing a mutation in the BRM1 site of icmR | This study |
| pOG-BRM2 | pOG-R125 containing a mutation in the BRM2 site of icmR | This study |
| pOG-BRR1 | icmR::lacZ fusion from pOG-R-125 in pBR322 | This study |
| pOG-BRR2 | pOG-BRR1 containing a single EcoRI site | This study |
| pOG-BRR3 | Ptac promoter in pOG-BRR2 | This study |
| pOG-cpxA-Km1 | pOG-U-cpxRA with the Kmr cassette in cpxA | This study |
| pOG-cpxA-Km2 | Insert of pOG-cpxA-Km1 in pLAW344 | This study |
| pOG-cpx-C2 | E. coli cpxR in pUC18 | This study |
| pOG-cpxR-1 | L. pneumophila cpxR in pUC18 | This study |
| pOG-cpxR-2 | pOG-cpxR-1 with the Kmr cassette in cpxR | This study |
| pOG-cpxR-3 | Insert of pOG-cpxR-2 in pLAW344 | This study |
| pOG-ECP2 | L. pneumophila cpxR in pET-15b | This study |
| pOG-R-125 | icmR::lacZ fusion in pGS-lac-01 | 50 |
| pOG-R-cpx3 | L. pneumophila cpxR in pOG-R125 | This study |
| pOG-RCPR3 | L. pneumophila cpxR in pOG-BRR3 | This study |
| pOG-R-cpxRA | L. pneumophila cpxRA in pOG-R125 | This study |
| pOG-rpoE-1 | L. pneumophila rpoE in pUC18 | This study |
| pOG-rpoE-2 | pOG-rpoE-1 with the Kmr cassette in rpoE | This study |
| pOG-rpoE-3 | Insert of pOG-rpoE-2 in pLAW344 | This study |
| pOG-RR16 | L. pneumophila genomic insert in pOG-BRR3 | This study |
| pOG-U-cpxRA | L. pneumophila cpxRA in pUC18 | This study |
| pOG-U-ECP | L. pneumophila cpxR in pUC18 | This study |
| pSS-R27 | Regulatory region of icmR in pMC1403 | 50 |
| pUC18 | oriR (ColE1) MCS Apr | 49 |
a
MCS, multiple cloning site.