Table 2.
Rinse and stimulus solutions for CT experiments
| KCl (mm) | Hepes (mm) | HCl (mm) | KHCO3 (mm) | KA (mm) | *CO2 (%) | AA (mm) | pH | |
|---|---|---|---|---|---|---|---|---|
| RHCl | 10 | — | — | — | — | — | — | — |
| SHCl | — | 20 | — | — | — | — | — | 1.7 |
| RAA | 175 | 10 | — | — | — | — | — | 6.1 |
| SAA | — | — | — | — | 175 | — | 10 | 6.1 |
| RCO2 | 72 | 10 | — | — | — | — | — | 7.4 |
| SCO2 | — | — | — | 72 | — | 10 | — | 7.4 |
R, rinse solution; S, stimulating solution; KA, potassium acetate; AA, acetic acid; Hepes, N-[2-hydroxyethyl]-piperazine-N′-[2-ethanesulphonic acid]
*
CO2/O2 (10/90%). In experiments with ionomycin all rinse solutions and stimulating solutions contained, in addition, 10mm CaCl2.