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. 2004 Apr 30;558(Pt 1):99–109. doi: 10.1113/jphysiol.2004.064899

Figure 1. Coimmunoprecipitation of SNAP-25 and VAMPs with hTRP1 in human platelets.

Figure 1

Resting platelets and platelets treated with 1 μm TG and 50 nm Iono were lysed and whole cell lysates were immunoprecipitated with anti-hTrp1 antibody. Proteins were separated by 15% SDS-PAGE and transferred to a nitrocellulose membrane for subsequent analysis. Membranes were either stained using Ponceau stain (top left panel) or analysed by Western blotting using either anti-SNAP-25 antibody (top middle panel), anti-VAMP antibody (top right panel) or anti-hTRPC1 antibody (bottom panels) as described in Methods. Positions of molecular mass markers are shown on the left. Arrows indicate the position of immunoreactive bands which shown increased detection or are only detected in TG + Iono treated cells. These results are representative of four independent experiments performed in different donors.