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. 2004 Apr 30;558(Pt 1):99–109. doi: 10.1113/jphysiol.2004.064899

Figure 5. Effect of botulinum toxin E on the maintenance of TG-induced Ca2+ entry in human platelets.

Figure 5

Fura-2-loaded human platelets were suspended in a Ca2+-free medium (200 μm EGTA added). Cells were then stimulated with TG (1 μm) and 3 min later 300 nm BoNT E or the vehicle (HBS; control) was added. CaCl2 (final concentration 300 μm) was added to the medium 1 h after the addition of the toxin or the vehicle to initiate Ca2+ entry. Data are presented as mean ±s.e.m. of five experiments performed in five different donors.