Figure 7. BoNT E reduces the coupling between hTRPC1 and the type II IP₃R in store-depleted human platelets.

Human platelets were pre-incubated in the absence or presence of BoNT E (300 nm) for 1 h at 37°C. Cells were then stimulated with TG (1 μm) plus Iono (50 nm) and then lysed. A, whole cell lysates were immunoprecipitated (i.p.) with anti-hTRPC1 antibody and analysed by Western blotting (WB) using anti-IP₃R type II antibody (anti-IP₃R II; top panel) or anti-hTRPC1 antibody (bottom panel). B, whole cell lysates were immunoprecipitated (i.p.) with anti-IP₃R II antibody and analysed by Western blotting (WB) using anti-hTRPC1 antibody (top panel) or anti-IP₃R II antibody (bottom panel). Positions of molecular mass markers are shown on the right. These results are representative of three independent experiments performed using different donors.