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. 2004 Jan 23;556(Pt 2):637–649. doi: 10.1113/jphysiol.2003.059956

Figure 2. Confocal fluorescence micrographs of bovine myocardium reconstituted with Rh-IA-labelled Tm and labelled with Fl-Ph.

Figure 2

The thin filament was first removed by gelsolin, and sequentially reconstituted with actin, followed by Rh-IA-labelled Tm. The preparation was then treated with Fl-conjugated phalloidin to label the actin filament. A, Fl fluorescence showing the distribution of the actin filament; B, Rh fluorescence showing the distribution of Tm; C, colocalization of the actin filament and Tm. Calibration bar, 10 μm.