Figure 6. Increased role of GPIb in platelet deposition and PS exposure upon reduced expression of GPVI.

Blood from wild-type and FcR γ-chain +/− and −/− mice was perfused over collagen (see Fig. 1). Wild-type and FcR γ-chain +/− blood were pre-incubated with 40 μg ml⁻¹ p0p/B Fab as indicated. Upper panels (A, C, E, G): representative phase contrast images (48 × 48 μm) after 4 min perfusion. Inserts are 200% magnifications showing platelet morphology. Lower panels (B, D, F, H): fluorescence images (150 × 150 μm) after staining with FITC-labelled annexin A5. I, surface area coverage of all platelets (white bars) and PS-exposing platelets (grey bars). Data are mean values ± s.e.m. (n = 3–5).