Figure 5. Ca²⁺ imaging in individual SMG acinar cell clusters and effect of CCh.

A–D, image of SMG acinar cell clusters and pseudo-colour images of F₃₄₀/F₃₈₀ under resting and CCh-stimulated (30 μm) conditions are shown (A, WT; B, M₁/M₅ double KO; C, M₃KO; D, M₁/M₃ double KO SMG). Scale bars indicate 100 μm. Lower panels in C and D show the [Ca²⁺]_i changes at regions 1–4 depicted in the upper panels. The results shown in A–D represent at least 8 separate measurements for each genotype. E, summarized responses of individual acinar cell clusters induced by 30 μm CCh in WT, M₁/M₅ double KO, M₃KO, or M₁/M₃ double KO SMG acinar cell clusters. Proportion of clusters categorized into three groups based on the responding area (0, 0–50 and 50–100%) as a precentage of the total number of clusters for each genotype was shown.