Figure 6. Cholinergically stimulated salivation in WT, M₁/M₅KO, M₃KO and M₁/M₃KO mice.

The saliva output in each 5 min period after stimulation is represented by the symbols and lines in the left panel (a) and the cumulative amount in 30 min in the right panel (b). A, salivation in response to 1 mg kg⁻¹ of pilocarpine (s.c.) in WT (n = 5), M₁/M₅ double KO (n = 4) and M₃KO (n = 5) mice. B, salivation in response to 10 mg kg⁻¹ of pilocarpine (s.c.) in WT (n = 5), M₃KO (n = 5), and M₁/M₃KO (n = 4) mice. The results are presented as the mean ± s.e.m.; some of the error bars are hidden by symbols. Statistical analysis was made using Scheffe's multiple comparisons following one-way ANOVA. In Aa, the values at all time points in M₃KO mice showed a statistically significant difference (P < 0.01), compared to WT or M₁/M₅ double KO mice. In Ba, significantly smaller values in M₃KO mice are depicted by asterisks (*P < 0.05, compared to WT), while the values at all time points in M₁/M₃ KO mice show statistically significant difference (P < 0.01, Student's t test), compared to WT or M₃KO mice. In b, significantly smaller values were depicted by asterisks (*P < 0.05 and **P < 0.01).