Table 2.
Rates of activation
| Cell Type | 0 mV τ (ms) (n) | Voltage (mV) | −(ΔG0 – zgEF)† (kJ mol−1) | τ (ms) (n) |
|---|---|---|---|---|
| WT | 240 ± 34 (6) | 100 | 49.8 | 27.6 ± 0.6 (3) |
| K525Q | 44 ± 9* (8) | 160 | 51.0 | 6.5 ± 0.5* (3) |
| R528Q | 1237 ± 54* (6) | 160 | 45.4 | 7.3 ± 3.4* (3) |
| R531Q | 4382 ± 541* (8) | 140 | 46.6 | 38.7 ± 2.4* (3) |
| R534Q | 113 ± 14* (7) | 140 | 50.0 | 3.9 ± 0.7* (3) |
| R537Q | 485 ± 133* (9) | 160 | 47.9 | 25.1 ± 5.4 (3) |
†
Total (chemical + electrostatic) potential energy was calculated from the ΔG0 and zgEF values derived from fitting eqn (2) to the steady-state activation data (see Table 1). Voltages shown in column 3 were chosen to give a total potential energy driving activation of ∼50 kJ mol−1 (shown in column four). The tau values shown in the fifth column were obtained at the corresponding voltages shown in column three. P < 0.05 compared to WT (ANOVA followed by Dunnett's method).