Table II.
Inhibition by simple sugars, sugar derivatives, and oligosaccharides of the interaction of Marpola with arcelin-1
| Inhibitory Sugar | Inhibition of the Interaction between Arcelin-1
and
|
|||
|---|---|---|---|---|
| Marpola
|
GNA
|
|||
| Percentage of inhibitiona | Relative inhibitory potency | Percentage of inhibitiona | Relative inhibitory potency | |
| Man | 8 | 1 | 5 | 1 |
| MeαMan | 65 | 8.12 | 12 | 2.4 |
| pNO2FαMan | 58 | 7.25 | ndb | nd |
| pNO2FβMan | 0 | 0 | nd | nd |
| Gal | 0 | 0 | 1 | 0.2 |
| Methyl α-d-galactopyranoside (MeαGal) | 0 | 0 | 1 | 0.2 |
| p-Nitrophenyl-α-galactopyranoside (pNO2FαGal) | 0 | 0 | nd | nd |
| p-Nitrophenyl-β-galactopyranoside (pNO2FβGal) | 1 | 0.12 | nd | nd |
| Glc | 3 | 0.37 | 2 | 0.4 |
| Methyl α-d-glucopyranoside (MeαGlc) | 35 | 4.37 | 3 | 0.6 |
| diManα1,2 | 19 | 2.37 | 11 | 2.2 |
| diManα1,3 | 21 | 2.62 | 39 | 7.8 |
| diManα1,4 | 1 | 0.12 | 6 | 1.2 |
| diManα1,6 | 16 | 2 | 13 | 2.6 |
| triManα1,3/1,6 | 31 | 3.87 | 60 | 12 |
| pentaManα1,3/1,6 | 54 | 6.75 | 97 | 19.4 |
All sugars were used at a concentration of 10 mm except pNO2FαMan (7 mm) and pNO2-phenyl-β-mannopyranoside (pNO2Fβ-Man, 2.5 mm).
a
Expressed as the percentage of lectin eluted from the arcelin-1-bound surface (as compared with measurements done in the absence of sugar). Results are the means of duplicate experiments.
b
nd, Not determined.