Figure 1. Ca²⁺ transients and caffeine-induced Ca²⁺ release.

A, intracellular [Ca²⁺] signals recorded 48 h after transfection with (i) Ad-LacZ (ii) Ad-FKBP12.6. B, a plot of caffeine-transient amplitude versus Ca²⁺ transient amplitude for individual cardiomyocytes from both Ad-LacZ (•) and Ad-FKBP12.6 (○) groups. Mean values (± s.d.) for both groups are shown by grey diamond symbols. The lines represent the best-fit linear correlation to the data: continuous line gradient (Ad-LacZ) = 0.50 ± 0.03(± s.d.); dashed line gradient (Ad-FKBP12.6) = 0.61 ± 0.07(± s.d.). C, time course of recovery of Ca²⁺ transient after a rest period; Ca²⁺ transients normalized to amplitude achieved in steady state; values are expressed as mean ± s.e.m., n = 20 in each group (○, Ad-FKBP12.6; •, Ad-LacZ).