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Western Blot analysis showed expression of nNOS (155 kDa) and eGFP (27 kDa) in wild-type, −EX and +EX; and nNOS+/−, −EX and +EX atria treated with Ad.nNOS or Ad.eGFP adenovirus. Ad.eGFP treatment was successful in causing atria to express eGFP protein. Ad.nNOS significantly increased atrial expression of nNOS protein in all groups (*P < 0.001, unpaired t test).
