Table I.

Novel mlo alleles

Allele	Mutant IDsa	Mutational Event at Mlo	Effect on MLO Protein	Protein Domain Affected	Bgh Resistance	Mutagen
mlo-12	Do 4122	C1396 → A	F240 → L	Second intracellular loop	Partial	Nitrosomethylurea
mlo-16	Do 2376	G1917 → Ab	Not applicable	Not applicable	Full	Ethymethane sulfonate (EMS)
mlo-27	Do 2021c	G2004 → A	G318 → E	Third intracellular loop	Full	EMS
mlo-28	Do 4228	C1224 → T	T222 → I	Second intracellular loop	Partial	NaN3
mlo-29	This study	C2051 → T	P334 → L	Third intracellular loop	Full	NaN3
mlo-30	Do 2234, 2235	A2242 → Td	Δ 6 amino acids	C terminus (intracellular)	Full	EMS

Nucleotide nos. based on the genomic Mlo DNA sequence starting from the translational start site (ATG).

^a According to Habekuss and Hentrich (1988). 

^b Nucleotide substitution in the conserved 3′ splice site of intron 9. 

^c Also Do 2002, 2003, 2004, 2005, 2014, 2015, 2016, and 2019. 

^d Nucleotide substitution in the conserved 3′ splice site of intron 11.