Changes in diameter (A–C) and intracellular Ca2+ (D–F) of endothelium-denuded arteries in physiological salt solution (PSS; Δ) compared to responses in [Ca2+]o plus 3 mm EGTA (▪) from 95 days gestation preterm fetal (A and D, n = 14), 140 days gestation near-term fetal (B and E; n = 12), and non-pregnant adult (C and F; n = 12) sheep. In all experiments, arteries were exposed to EGTA solution for 20 min before determining [Ca2+]o plus 3 mm EGTA responses to pressure. Values are means ± s.e.m. *P < 0.05 compared to respective [Ca2+]o plus 3 mm EGTA response, by ANOVA.