A, Northern example for one subject before training (Pre), after 30 (T30) and 90 (T90) days of training and following 3 (D3), 10 (D10), 30 (D30), 60 (D60) and 90 (D90) days of detraining. B, staining of the ribosomal RNA with SYBR green before blotting is shown below.