A, UTP had no effect on the spontaneous currents; however, this cell could respond to ATPγS. B, α,β-meATP evoked transient facilitation of both sEPSC and sIPSC frequency. α,β-meATP was applied during the period indicated by the horizontal bars. C, overlaid traces (5 sweeps) are shown for control (i), during the application of 100 μmα,β-meATP (ii) and after wash (iii). D, summary data for the effect of α,β-meATP on the frequency of sEPSCs (left) and sIPSCs (right). Each column shows the frequency in control (i), on application of 100 μmα,β-meATP (ii) and after wash (iii). Each column shows the mean from 7 (sEPSC) and 4 (sIPSC) cells (including only those cells responding to α,β-meATP).