Figure 6.

Constitutive expression of CBF1 increases CAT1 and GST-like transcripts and CAT activity and reduces the H₂O₂ concentration in the transgenic plants. Total RNA (10 μg) was extracted from leaves of wild-type and transgenic tomato plant C15 line grown at 24°C (N) and was exposed to chilling for 3 d (C), respectively. The northern blots were hybridized with the probes for CAT1 and β-TUBULIN (A). The activities of CAT (B) and H₂O₂ concentration (C) in the transgenic tomato plants are shown. Tomato plants were grown at 24°C growth condition (control) and exposed to 0°C for 7 d (chilling), respectively. The hydrogen peroxide concentration was also measured in the same condition.