Figure 3. Effects of preapplication of combined toxin treatment on WIN55,212-2-mediated inhibition of presynaptic calcium influx.
Time course of fluo-4FF fluorescence transients before, during and after bath application of combined blockers (ω-agatoxin TK (250 nm), ω-conotoxin GVIA (250 nm) and SNX-482 (100 nm)) followed by application of WIN55,212-2 (1 μm) (n = 4). The insets show the fluorescence transients in one of these experiments (top) and the WIN55,212-2-mediated reduction of the fluorescence transients normalized to the plateau level obtained after combined toxin treatment (bottom).