Figure 2.

Suppression of TSS enzyme activity in elicitor-treated tobacco cell suspension cultures is accompanied by changes in the level of the TSS protein and mRNA. Cells were collected at the indicated time points after addition of 1 μg of P. parasitica elicitin per milliliter of cell suspension culture and kept frozen until analyzed for TSS enzyme activity (A), TSS protein (B), and TSS mRNA (C). Microsomes isolated from the cells were used to measure SS enzyme activity and to quantify TSS protein by chemiluminescent immunodetection, whereas TSS mRNA was detected by RNA-blot hybridization using a full-length cDNA to probe total RNA isolated from aliquots of the collected cells.