Figure 5.

Analysis of 1-month-old wild-type tobacco plant tissues for TSS enzyme activity and protein levels. A 1-month-old wild-type tobacco plant (A) was dissected into its component parts as indicated (B and C), and the level of TSS enzyme activity (D) and enzyme protein (E) was determined. Dashed lines indicate how organs were excised into specific tissues; LB, leaf blade; LM, leaf margin; PT, petiole; AM, apical meristem; ST, stem; and RT, roots. The inset bars in A through C equal 1 cm. Microsomes prepared from each of the tissues were used simultaneously to measure TSS enzyme activity (D) and the level of the TSS protein by chemiluminescent immunodetection (E).