Table 1. Values of parameters used by the release flux algorithm and simulations.
| Parameter | Value | Parameter | value |
|---|---|---|---|
| Fluo:Ca ON rate (107m−1 s−1) | 26.7 | Parv:Ca ON-rate (107m−1 s−1) | 12.5 |
| Fluo:Ca OFF rate (s−1) | 136 | Parv:Ca OFF-rate (s−1) | 0.5 |
| P-Fluo:Ca ON rate (107m−1 s−1) | 1.72 | Parv:Mg ON-rate (107m−1 s−1) | 0.003 |
| P-Fluo:Ca OFF rate (s−1) | 33 | Parv:Mg OFF-rate (s−1) | 3 |
| Fluo:P ON rate (107m−1 s−1) | 1.15 | SO4:Ca ON-rate (107m−1 s−1) | 50 |
| Fluo:P OFF rate (104 s−1) | 0.42 | SO4:Ca OFF-rate (107 s−1) | 2.5 |
| Ca-Fluo:P ON rate (107m−1 s−1) | 1.15 | Maximum pump rate (mm s−1) | 9.8 |
| Ca-Fluo:P OFF rate (104m−1 s−1) | 1.59 | DCa (10−7 cm2 s−1) | 35 |
| [P]+[P:Fluo] (mm) | 3 | Dfluo (10−7 cm2 s−1) | 15 |
| EGTA:Ca ON rate (107m−1 s−1) | 0.2 | DATP (10−7 cm2 s−1) | 14 |
| EGTA:Ca OFF rate (s−1) | 2 | DSO4 (10−7 cm2 s−1) | 18 |
| Trop:Ca ON-rate (107m−1 s−1) | 20.3 | Dparv (10−7 cm2 s−1) | 0.16 |
| Trop-Ca:Ca ON-rate (107m−1 s−1) | 10.2 | DEGTA (10−7 cm2 s−1) | 0.36 |
| Trop:Ca OFF-rate (104 s−1) | 2.64 | [Troponin sites] (mm) | 0.36 |
| Trop-Ca:Ca OFF-rate (s−1) | 13.2 | [Parvalbumin] (mm) | 1 |
| ATP:Ca ON-rate (107m−1 s−1) | 15 | [Pump sites] (mm) | 0.24 |
| ATP:Ca OFF-rate (104 s−1) | 3 | [EGTA] (mm) | 1 |
| ATP:Mg ON-rate (107m−1 s−1) | 0.195 | [ATP] (mm) | 5 |
| ATP:Mg OFF-rate (s−1) | 195 | [SO42−] (mm) | 80 |
Model and parameters are as in Zhou et al. (2003a) with two modifications: ternary reactions take place among dye, Ca2+ and cell sites ‘P’ (Baylor et al. 2002). Additionally, the reaction between troponin and Ca2+ was described by a two-step cooperative scheme, whereby binding of one Ca2+ leads to an increase in affinity for a second ion (Baylor et al. 2002). Parv, parvalbumin. Trop, troponin. [Ca2+] was 0.05 μm[Mg2+] was 2 mm. In ternary reactions, the colon identifies the binding ligands. Thus, ‘P-Fluo:Ca’ designates rates of Ca2+ binding to site-bound dye, etc.