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. 2004 Mar 5;557(Pt 1):59–75. doi: 10.1113/jphysiol.2004.061291

Fig. 1. Two-pipette stimulating and recording method.

Fig. 1

A, 10 × phase contrast image of a normal FDB fibre impaled with two electrodes as described in text. B, 40 × magnification images of same fibre showing phase image and corresponding OGB-5N fluorescence image from the region circumscribed by the white square in A. Calibration bar is 30μm. C, surface AP from a normal mouse FDB from pipette 1 (black) and pipette 2 (grey). Internal solution contained 5mm EGTA and the external solution was normal Tyrode solution.