Table 2. OGB-5N transient parameters and peak [Ca2+] for normal and mdx muscle fibres in the absence of EGTA.
(ΔF/F)peak | Peak [Ca2+] (μm) | tp (ms) | FDHM (ms) | τ1 (ms) | τ2 (ms) | Aslow/Afast | |
---|---|---|---|---|---|---|---|
Normal FDB | 1.1 ± 0.02 | 6.0 ± 0.1 | 2.1 ± 0.1 | 8.0 ± 0.5 | 2.3 ± 0.1 | 37 ± 3 | 1.3 ± 0.1 |
mdx FDB | 0.64 ± 0.04 | 3.3 ± 0.2 | 2.5 ± 0.1 | 48 ± 6 | 3.0 ± 0.4 | 113 ± 18 | 2.5 ± 0.3 |
For experiments, fluorescence transient parameters and peak [Ca2+] were obtained from n= 5 FDB fibres from 2 normal mice and n= 11 fibres from 2 mdx mice. All transients were obtained with 5 mm total [Mg2+].
Statistical significance between normal and mdx FDB fibres (P < 0.05);
statistical significance between normal and mdx FDB fibres (P < 0.0001)