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. 2004 Jul 2;559(Pt 1):67–84. doi: 10.1113/jphysiol.2004.066944

Figure 9. Effects of wortmannin and removal of ATP.

Figure 9

A and B, representative responses to oxo-M after preincubating with 1 μm or 50 μm wortmannin (WMN1 and WMN50). C, averaged time courses of muscarinic suppression and recovery after preincubation with 1 μm or 50 μm wortmannin (n = 5 for each). These data have been corrected for rundown and normalized (see Methods). D, summary of extent of inhibition with wortmannin or with 4 mm of the non-hydrolysable AMP-PCP replacing all pipette ATP. E, averaged time course of muscarinic suppression and recovery from inhibition with 4 mm AMP-PCP included in the pipette instead of ATP, corrected and normalized as in C. F, recovery from muscarinic inhibition measured with correction for rundown 3 min after maximal oxo-M inhibition with wortmannin or ATP-free pipette solution. *P≤ 0.05.