Figure 6. Effect of hypoxia on Kv currents in the presence of TEA.

The graph shows the peak current amplitude in depolarizing pulses to +40 mV applied every 5 s. In this cell, hypoxic exposure was applied three times, during the indicated periods. The second hypoxic application was performed while the cell was superfused with a solution containing 10 μm TEA as indicated in the graph. Selected traces during the experiment (labelled with numbers 1–9), and the subtracted hypoxia-sensitive fractions (labelled d) are shown in the upper part of the figure. The right panel shows a bar plot with the average results obtained from six cells in which the same protocol was applied. In these cells, hypoxia alone produced a reduction in the peak current amplitude to an 86% of the initial value (white bar), but no effect of hypoxia was seen in the presence of TEA (grey bar), indicating that the hypoxia-sensitive portion of the current is blocked with this low TEA concentrations. **P < 0.01.