Figure 7. Amplification products for Kv subunits obtained from GFP-positive cells' mRNA.

Transcript detection of the different Kv subunits was performed in RT products from pooled GFP-positive cells from mouse CB cultures. Positive control lanes (C+) show the expression of the corresponding Kv in mouse cortex. The M-number labels indicate the GFP-expressing cells' pool number used for each amplification. In the cases in which Kv transcripts were not detected in the cells (i.e. Kv2.1), control amplifications to demonstrate the detection of an ubiquitous transcript (β-actin) were performed. Amplifications were generally done with 3 mm Mg²⁺, except for Kv2.2, which was done with 1.5 mm Mg²⁺. The expected size of the PCR products was 595 bp (Kv2.1), 482 bp (β-actin), 359 bp (Kv2.2), 627 bp (Kv3.1), 575 bp (Kv3.2) and 378 bp (Kv3.3). The marks on the right of each gel correspond to molecular weights of 300, 500, 700 and 1000 bp as shown in the last image.