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. 2004 Mar 19;557(Pt 2):457–471. doi: 10.1113/jphysiol.2004.062281

Figure 8. Immunofluorescence labelling in mouse CB chemoreceptor cells.

Figure 8

A, the presence of Kv3.2 and Kv3.1b in TH-expressing cells was shown both by immunofluorescence labelling of TH–GFP cells and by double labelling with TH and the corresponding anti-Kv antibody. On the contrary, Kv2.1 labelling was mainly found in GFP-negative cells, although colocalization could occasionally be detected (see arrow in the lower panels). B, Kv3.4 is not present in GFP-positive cells; there is no overlap of green and red fluorochromes in the merged panels. The change in the morphology of the Kv3.4-positive cells is evidenced by comparing a 1-day culture (upper panel) and a 5-day culture (lower panel). C, immunofluorescence labelling of Kv3.3 in mouse CB chemoreceptor cells shows the expression of Kv3.3 in every GFP-positive cell (yellow in the merged panels). The specificity of the Kv3.3 ubiquitous labelling was confirmed by the loss of Kv3.3 tagging in cells preincubated with the control peptide (CP). In this case, there is no colour change in the merged panel compared to the TH/GFP panel.