Figure 2. Regions needed for the functional complementation between N_1–720 and C_721–988.

A, schematic representation of the carboxy-terminal region of ClC-1 that was analysed by mutagenesis. B, conductance levels (at 0 mV, in μS) of key constructs after expression in Xenopus oocytes. C, biochemical analysis of proteins analysed electrophysiologically in A and indicated below the different lanes. Western blot analysis of oocyte membranes for HA-N_1–720 using the 3F10 antibody against HA epitope (top panel), and for myc-C_721–988 constructs using the 9E10 antibody against the myc epitope (centre panel). Lower panel, immunoprecipitation of solubilized oocyte membranes using the HA antibody for precipitation and the myc antibody for detection in Western blotting. Three independent experiments gave similar results.