Figure 2. RT-PCR on purified α- and β-cells with specific primers against different GABA_BR subunits.

A, total RNA from rat islets as well as FACS-purified α- and β-cells was reverse transcribed into cDNA. PCR analysis was performed using specific primers against GABA_BR_1a-d,f, GABA_BR_1a,f, GABA_BR_1c,f, GABA_BR_1d and GABA_BR₂ (+). In the negative control reaction (−) reverse transcriptase was omitted. Molecular standards are shown to the left and the right. B, multiplex RT-PCR on islets (isl) and purified α- and β-cell with specific primers against insulin and glucagon.