Figure 8. Baclofen does not affect glucose-induced intracellular Ca²⁺ signalling and electrical activity of β-cells.

A, [Ca²⁺]_i was measured in an intact islet by dual-wavelength microfluorimetry using fura-2 AM as the Ca²⁺ indicator. [Ca²⁺]_i responses are expressed as fura-2 fluorescence (F) ratios (F₃₅₀/F₃₈₀). The islet was superfused with a solution containing 15 mm glucose to generate the characteristic biphasic [Ca²⁺]_i response. At the time indicated, 10 μm baclofen was included in the bath solution. The trace is representative of nine experiments. B, membrane potential recording from a β-cell in an intact rat islet obtained using the perforated-patch whole-cell configuration. Electrical activity was induced by addition of 20 mm glucose to the bath solution. Baclofen (10 μm) was applied as indicated by the horizontal line.