Figure 4. Lack of buffer effects on mucin secretion in SLO-permeabilized SPOC1 cells.

A, cells were exposed to high Ca²⁺ (3 μm), buffered by EGTA or BAPTA (0.1 μm Ca²⁺), ± the PKC inhibitors BIMII (1 μm) and calphostin C (1 μm; n = 4 SPOC1 passages). B, cells were exposed to IP₃ (0.3 μm) and buffered by either EGTA or BAPTA (0.03–10 mm) at a constant 0.1 μm Ca²⁺ (n = 4 SPOC1 passages).