Figure 3. Effect of PMA and chelerythrine on the potentiation of K⁺-induced amine release produced by CCCP.

Secretion peaks in nA obtained during 5 s pulses of depolarizing concentrations (150 mm) of K⁺ (dots at the bottom of the panels). A, PMA (100 nm) and CCCP (2 μm) were perfused 5 min and 90 s, respectively, prior to the high K⁺ challenges. B, chelerythrine (1 μm) was present in the medium 8.5 min prior to and during the 90 s period of perfusion with CCCP. C, potentiation of high K⁺-evoked amine release by CCCP (n = 36), PMA (n = 6), CCCP after PMA (n = 5) and CCCP in the presence of chelerythrine (n = 6). Bars represent mean ± s.e.m. *P < 0.01 versus CCCP. Other conditions were as described in legend to Fig. 1.