Figure 2.

DNA-blot analysis of glyphosate-sensitive and -resistant goosegrass individuals. Ten micrograms of Genomic DNA from five sensitive individuals (samples S1–S5) and five resistant individuals (samples R1–R5) were digested with either EcoRV, SphI, SspI, or ScaI as indicated above, then size fractionated on 0.8% (w/v) agarose gels and transferred to nylon membranes. Blots were then hybridized with an L. rigidum ³²P-labeled EPSPS cDNA, washed at high stringency (see “Materials and Methods”), then subjected to autoradiography.