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IC50 (glyphosate) determinations for the (R) and (S) biotype EPSPS activities. Radiometric EPSPS assays were performed on (R) and (S) biotype crown tissue extracts in the presence of glyphosate at concentrations ranging from 0.1 to 100 μm. Inhibition curves and IC50 (inflection point) values were generated by nonlinear regression analysis using the GraFit software package (Leatherbarrow, 1998).
