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. 2002 Jul;129(3):1265–1275. doi: 10.1104/pp.001560

Figure 5.

Figure 5

DNA cassettes containing different EPSPS mature protein coding regions for E. coli expression studies. P-lac and lac-O represent the native promoter and operator sequence from the E. coli lac operon. The Shine/Dalgarno sequence is indicated by the letters S/D. The predicted EPSPS mature coding region from the resistant biotype is indicated by (R) and the sensitive biotype EPSPS by (S). P106S is an engineered variant of (R) containing a Pro at position 381; P381L is a an engineered variant of (R) containing a Pro at position 106; “ter” represents the rho-independent transcriptional terminator element of the E. coli trpA gene (Sato et al., 1987).