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. 2002 Jul;129(3):1265–1275. doi: 10.1104/pp.001560

Figure 6.

Figure 6

Glyphosate sensitivity of pMON45364- and pMON45365-transformed cell lines. Overnight cultures of E. coli SR481 (aroA−) cell lines harboring pMON45364 [containing the EPSPS cDNA from the (R) biotype], and pMON45365 [containing the EPSPS cDNA from the (S) biotype], were used to inoculate 3.0-mL cultures containing minimal media supplemented with either 0.0, 0.5, 1.5, or 5.0 mm glyphosate. Aliquots were removed at t = 0, 24, 28, 32, and 48 h and optical densities were monitored at 595 nm. Before inoculation, optical density measurements were taken from the original overnight cultures to confirm similar cell densities. Each data point represents the mean of three replicates; error bars indicate sd. Where not visible, the error is enclosed within the data symbol. C, Growth rates of cells harboring pMON45337 (“empty” vector control). a, Growth rates of cells expressing the mature (S) EPSPS from pMON45365. b, Growth rates of cells expressing the mature (R) EPSPS from pMON45364.