Table I.
Kinetic parameters for E. coli-expressed enzymes
| Construct | EPSPS | IC50 | Km(app)(PEP) | Ki(app)(Glyphosate) |
|---|---|---|---|---|
| μm | nm | |||
| pMON45364 | Resistant | 30.4 ± 2.7 | 7.0 ± 1.0 | 759 ± 140 |
| pMON45370 | P106S | 38.2 ± 7.6 | 8.9 ± 1.0 | 1,040 ± 138 |
| pMON45365 | Sensitive | 6.3 ± 0.9 | 3.8 ± 1.1 | 47.8 ± 16 |
| pMON45371 | P381L | 8.6 ± 1.3 | 3.2 ± 0.5 | 29.2 ± 5.1 |
Kinetic constants were determined as described previously (Padgette et al., 1991) using triplicate assays for all experiments. Calculations were performed using the GraFit software package (Leatherbarrow, 1998). IC50, Km(app)(PEP), and Ki(app)(glyphosate) values are expressed as ± se derived from the best fit.