Figure 7. Effect of desensitization of PAC1-Rs and VPAC-Rs on a single IMG neurone on colonic distension-evoked neuronal response.

A, control. B, after desensitization. Receptor desensitization was attained by ganglion preincubation for 20 min with PACAP27 (500 μm). Period of colonic distension of 10 cm H₂O is marked with arrows. The bottom diagrams indicate the frequency of the F-EPSPs with amplitude greater then 3 mV and of the APs counted in 5 s bins from the electrical recordings in A and B. NKS, normal Krebs solution.